Bioaktivitas Larvasida Ekstrak Formula Daun Jeruk Nipis (Citrus aurantifolia) Dengan Jeruk Purut (Citrus hystrix) Terhadap Nyamuk Aedes aegypti

Sofiya Anggi Salbia; Emantis Rosa; Endah Setyaningrum; Hendri Busman

Authors

Sofiya Anggi Salbia Universitas Lampung
Emantis Rosa Universitas Lampung
Endah Setyaningrum Universitas Lampung
Hendri Busman Universitas Lampung

Keywords:

Aedes aegypti, Biolarvasida, Citrus aurantifolia, Citrus hystrix, Fitokimia, Morfologi

Abstract

Dengue Hemorrhagic Fever (DHF), transmitted by Aedes aegypti mosquitoes, remains a public health problem, while the use of synthetic insecticides carries the risk of resistance and environmental pollution. This research aimed to identify the secondary metabolites in lime (Citrus aurantifolia) and kaffir lime (Citrus hystrix) leaf extracts, to determine the most effective extract combination for producing the highest mortality of third-instar Aedes aegypti larvae, and to observe the resulting morphological changes. The research employed a Completely Randomized Design (CRD) with four treatments: a control (distilled water), F1 (10 mL of lime leaf extract + 10 mL of kaffir lime leaf extract), F2 (20 mL of lime leaf extract + 20 mL of kaffir lime leaf extract), and F3 (30 mL of lime leaf extract + 30 mL of kaffir lime leaf extract). Larval mortality percentages were analyzed using the non-parametric Kruskal-Wallis test, followed by Dunn’s post hoc test. Phytochemical screening revealed the presence of alkaloids, flavonoids, tannins, and saponins. The results showed that the treatment containing 30 mL of lime leaf extract + 30 mL of kaffir lime leaf extract was the most effective, with a mean mortality of 95.40%. Morphological observations showed that increasing extract concentrations caused structural damage to Aedes aegypti larvae.

References

Adrianto, H., Yotopranoto, S., & Hamidah, H. (2014). Efektivitas ekstrak daun jeruk purut, jeruk limau, dan jeruk bali terhadap larva Aedes aegypti. Aspirator, 6(1), 1–6.

Aini, F. Q., et al. (2024). Identifikasi karakteristik morfologi dan kandungan famili Rutaceae. J. Teknool. Pangan & Tani, 2(2), 46–55.

Anggraeni Putri, P., Chatri, M., & Advinda, L. (2023). Characteristics of saponin secondary metabolite compounds in plants. Serambi Biologi, 8(2), 251–258.

Arifin, B., & Ibrahim, S. (2018). Struktur, bioaktivitas dan antioksidan flavonoid. Jurnal Zarah, 6(1), 21–29.

Bawekes, S. M., Yudistira, A., & Rumondor, E. M. (2023). Uji kualitatif kandungan senyawa kimia perasan jeruk nipis. PHARMACON, 12(3), 373–377.

Berliani, J. R., et al. (2021). Efektivitas larvasida formula granul ekstrak bunga melati dan biji pepaya. JPSCR: Journal of Pharmaceutical Science and Clinical Research, 6(1), 45–55.

Dinata, A. (2017). Biopestisida: Pemanfaatan bahan alam sebagai pengendali hama. Penebar Swadaya.

Hersila, S., et al. (2023). Senyawa metabolit sekunder (tanin) pada tanaman sebagai antifungi. Jurnal Embrio, 15(1), 16–22.

Hidayani, W. R. (2021). Demam berdarah dengue: Perilaku rumah tangga & penanggulangan. CV. Pena Persada.

Kumara, C. J. (2021). Efektivitas flavonoid, tanin, saponin dan alkaloid terhadap mortalitas larva Aedes aegypti [Skripsi, Universitas Muhammadiyah Surakarta].

Prasetyo, B., & Lestari, P. (2020). Mekanisme Racun Saraf Alkaloid Tumbuhan Terhadap Mortalitas Larva Aedes aegypti. Jurnal Entomologi Indonesia, 17(3), 112–119.

Purwati, A., Dewatisari, W. F., & Pangestu, A. (2023). Identifikasi Kandungan Metabolit Sekunder Daun Akar Wangi (Vetiveria zizanioides (L.)). Partner, 29(2), 149–158.

Susilowati, R. P., & Sari, M. P. (2022). Histopathological changes of midgut epithelial cells of Aedes aegypti larvae exposed to permot leaf extract (Passiflora foetida). Jurnal Pembelajaran dan Biologi Nukleus, 8(1), 53–63.

Utami, W. W., Ahmad, A. R., & Malik, A. (2016). Uji aktivitas larvasida ekstrak daun jarak kepyar (Ricinus communis L.) terhadap larva nyamuk Aedes aegypti. Jurnal Fitofarmaka Indonesia, 3(1), 141–145.