Respons Pertumbuhan Planlet Kentang (Solanum tuberosum L.) Terhadap Aplikasi Ekstrak Kecambah Kacang Hijau (Vigna radiata L.) Secara In Vitro
Keywords:
Growth, In Vitro, PGR, Solanum tuberosum, Vigna radiataAbstract
Potato (Solanum tuberosum L.) is an important food commodity with high economic value; however, the production of quality seedlings still faces obstacles due to the risk of pathogen contamination and the limitations of conventional propagation methods. In vitro tissue culture is an effective solution, but it remains dependent on synthetic plant growth regulators (PGRs) that are expensive and potentially harmful to the environment. Mung bean (Vigna radiata L.) sprout extract can serve as an alternative natural PGR that is more environmentally friendly. This study aimed to determine the effect of mung bean sprout extract as a natural PGR on the in vitro growth of potato planlets and to identify the most optimal concentration. The study was designed using a Completely Randomized Design (CRD) with five concentration levels of mung bean sprout extract: 0% (control), 2%, 4%, 6%, and 8%, each with five replicates. Parameters observed included the percentage of live planlets, planlet visualization, planlet height, number of leaves, root length, and stomatal density two weeks after planting. Data were analyzed using Analysis of Variance (ANOVA) followed by Tukey's HSD test at a 5% significance level. The results showed that all planlets survived across all treatments at 100%. The application of mung bean sprout extract had no significant effect on planlet height, number of leaves, and root length, but significantly affected stomatal density. A concentration of 4% produced the highest stomatal density at 265.35 stomata/mm².
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