Penguatan Kompetensi Guru Biologi Melalui Pelatihan Teknik Kultur Jaringan Tumbuhan Di Kabupaten Tanggamus, Lampung
Keywords:
Guru Biologi, Kultur Jaringan Tumbuhan, Kompetensi, Pelatihan, Pembelajaran Berbasis PraktikumAbstract
Biology teachers' competence in utilizing laboratory facilities as learning media plays an important role in developing students' scientific skills. However, the application of plant tissue culture techniques as a practical component of biotechnology learning is still limited in many secondary schools. This community service program aimed to strengthen the competence of senior high school Biology teachers through plant tissue culture training conducted at SMAN 1 Wonosobo, Tanggamus Regency, Lampung. The program consisted of lectures, demonstrations, hands-on laboratory practice, and learning evaluation using pre-test and post-test assessments. The training materials covered the basic principles of plant tissue culture, sterilization techniques, culture media preparation, explant inoculation, subculturing, and plantlet maintenance. The evaluation results showed that the participants' average knowledge score increased from 39.8% before the training to 88.0% after the training, representing an improvement of 48.2 percentage points. These findings indicate that the training program was effective in enhancing teachers' knowledge and practical skills in plant tissue culture techniques. The improved competence is expected to support the implementation of laboratory-based biology learning and promote the development of students' scientific skills in secondary schools.
References
Akin-Idowu, P. E., Ibitoye, D. O., & Ademoyegun, O. T. (2009). Tissue culture as a plant production technique for horticultural crops. African Journal of Biotechnology, 8(16), 3782–3788.
Direktorat Jenderal Pendidikan Anak Usia Dini, Pendidikan Dasar dan Pendidikan Menengah. (2020). Data Pokok SMA Negeri 1 Wonosobo Kecamatan Wonosobo Kabupaten Tanggamus Provinsi Lampung. https://dapo.kemdikbud.go.id/sekolah/C679626E512E5EFA48D1
Eriansyah, M., Susiyanti, & Putra, Y. (2014). Pengaruh pemotongan eksplan dan pemberian beberapa konsentrasi air kelapa terhadap pertumbuhan dan perkembangan eksplan pisang ketan (Musa paradisiaca) secara in vitro. Agrologia, 3(1), 54–61.
Gonzáles, R. G., Quiroz, K., Carrasco, B., & Caligari, P. D. S. (2010). Plant tissue culture: Current status, opportunities and challenges. Ciencia e Investigación Agraria, 37(3), 5–30.
Kemendikbud RI. (2020). Jumlah data satuan pendidikan (sekolah) per kabupaten/kota. https://referensi.data.kemdikbud.go.id/index11.php?kode=120600&level=2
Liu, E., Liu, C., & Wang, J. (2015). Pre-service science teacher preparation in China: Challenges and promises. Journal of Science Teacher Education, 26(1), 29–44. https://doi.org/10.1007/s10972-014-9404-1
Nurcahyani, E., Agustrina, R., Suroso, E., & Andari, G. (2016). Analysis of peroxidase enzyme and total phenol from Spathoglottis plicata Bl. as a result of in vitro fusaric acid selection toward Fusarium oxysporum. International Journal of Applied Agricultural Science, 2(6), 79–82.
Nurcahyani, E., Sumardi, I., Hadisutrisno, B., & Suharyanto, E. (2012). Penekanan penyakit busuk batang vanili (Fusarium oxysporum f. sp. vanillae) melalui seleksi asam fusarat secara in vitro. Jurnal Hama dan Penyakit Tumbuhan Tropika, 12(1), 12–22.
Nurcahyani, E., Sumardi, I., Hadisutrisno, B., & Suharyanto, E. (2017). DNA pattern analysis of Vanilla planifolia Andrew plantlets resistant to Fusarium oxysporum f. sp. vanillae. World Journal of Pharmaceutical and Life Sciences, 3(4), 27–34.
Nurcahyani, E., Sumardi, Irawan, B., Sari, E. Y., & Sari, T. L. (2019). In vitro study: Induced resistance of cassava (Manihot esculenta Crantz) plantlets against Fusarium oxysporum based on analysis of phenol content. World Journal of Pharmaceutical and Life Sciences, 5(2), 195–198.
Nurcahyani, E., Sumardi, Qudus, H. I., Wahyuningsih, S., Palupi, A., & Sholekhah. (2020). In vitro selection of Phalaenopsis amabilis (L.) Bl. plantlets resulting from induced resistance with fusaric acid. World Journal of Pharmaceutical and Life Sciences, 6(2), 25–28.
Rustaman, N. Y. (2005). Perkembangan penelitian pembelajaran berbasis inkuiri dalam pendidikan sains. Dalam Prosiding Seminar Nasional II Himpunan Ikatan Sarjana dan Pemerhati Pendidikan IPA Indonesia (hlm. 1–21). Fakultas Pendidikan Matematika dan Ilmu Pengetahuan Alam, Universitas Pendidikan Indonesia.
Yuliarti, N. (2010). Kultur jaringan tanaman skala rumah tangga. Lily Publisher.
