Efek Kuning Telur Terhadap Motilitas Sperma Ikan Lukas (Puntius bramoides Val) Pascakriopreservasi

Primasari  Pertiwi; Abinawanto; Salman  Farisi

Authors

Primasari Pertiwi Universitas Lampung
Abinawanto Universitas Lampung
Salman Farisi Universitas Lampung

Keywords:

Ikan Lukas, Motilitas, Kriopreservasi, Kuning Telur

Abstract

The research aims to determine the effect of several concentrations of egg yolk as an effective natural cryoprotectant for cryopreservation and to analyze the quality of lukas fish spermatozoa (Puntius bramoides Val) 48 hours after cryopreservation. This research used a 9 treatments and 4 replications. The concentration of egg yolk used as a cryoprotectant was 0% (negative control), 5%, 7%, 9%, 11%, 13%, 15%, 17%, and 1% Carboxymethyl Cellulose (CMC) was used as a positive control. The results showed that the use of several concentrations of egg yolk as a natural extracellular cryoprotectant was able to significantly influence (P<0.005) the quality of Lukas fish spermatozoa 48 hours after cryopreservation. The use of egg yolk concentration of 9% is the optimum concentration in maintaining the motility of Lukas fish spermatozoa with the average percentage obtained each being 80.45 ± 0.93%.

References

Abinawanto, S. Rahayu, R. Lestari. (2013). Cryopreservation of Java barb (Barbonymus gonionotus) spermatozoa using egg yolk as a cryoprotectant. Global Veterinary 10(3): 318--321.

Arifiantini, R. I., T. L. Yusuf & O. Indah. (2005). Kaji banding dua teknik pengemasan menggunakan tiga macam pengencer untuk pembekuan semen sapi Friesian Holstein (FH). Seminar Nasional Teknologi Perternakan dan Veteriner. 366--376.

Bearden, H.J., J.W. Fuquay & S.T. Willard. (2004). Applied animal reproduction. 6th ed. Pearson Prentice Hall, New Jersey: xvi + 427 hlm.

Bergeron, A., M.H. Crete, Y. Brindel & P. Manjunath. (2004). Low-density lipoprotein fraction from hen’s egg yolk decreases the binding of the major proteins of bovine seminal plasma to sperm and prevents lipid efflux from the sperm membrane. Biology of Reproduction 70: 708--717.

Fujaya, Y. (2002). Fisiologi ikan: Dasar pengembangan teknologi perikanan. Fakultas Ilmu Kelautan dan Perikanan Universitas Hasanudin, Makassar: vii + 204 hlm.

Kasim, K., C. Umar, P.S. Sulaiman & N. Zulfia. (2012). Makanan dan reproduksi ikan lukas (Dangila cuvieri, Valenciennes 1842) di perairan waduk gajah mugkur Wonogiri. BAWAL 4(2): 113--120.

Routray, P., D. K. Verma, S. K, Sarkar & N. Sarangi. (2007). Recent advances in carp seed production and milt cryopreservation. Fish Physiology Biochemistry 10: 1--15.

Rurangwa, E., D. E. Kime, F. Ollevier & J. P. Nash. (2004). The measurement of sperm motility and factors affecting sperm quality in cultured fish. Aquaculture 234: 1--28.

Setyono, B. (2009). Pengaruh Perbedaan Konsentrasi Bahan pada Pengencer Sperma Ikan “Skim Kuning Telur” terhadap Laju Fertilisasi, Laju Penetasan dan Sintasan Ikan Mas (Cyprinus carpio L). Gamma 5(1): 01--12.

Sularto, E. Tahapari, W. Hadie & I. Nurlela. (2013). Penggunaan kuning telur bebek sebagai ekstender pada proses kriopreservasi sperma ikan patin jambal. Konferensi Akuakultur Indonesia 220--225.

Sukumasavin, N. (2008). Advanced freshwater aquaculture: Fish reproduction. Inland Fisheries Research and Development Bureuau, Bepartement of Fisheries, Thailand: 133--159 hlm.

Sunarma, A. (2007). Kriopreservasi sperma ikan nilem (Osteochilus hasselti) menggunakan ekstender dan krioprotektan berbeda. Tesis S2-Biologi Universitas Jenderal Soedirman, Purwokerto: xvii + 55 hlm.

Umiyati, T. (2005). Studi Pakan Alami Ikan Senggaringan (Mystus nigriceps) dan Ikan Lukas (Puntius bramoides) di Perairan Bendung Gerak Serayu Kabupaten Banyumas. Skripsi (Tidak Dipublikasi). Fakultas Biologi Universitas Jenderal Soedirman. Purwokerto.

Vishwanath, R. & P. Shannon. (2000). Storage of Bovine Semen in Liquid Frozen State. Animal Reproduction Science 62: 23--53.